The mechanism of treating effects of LIPUS on hematopoietic damage will be tried to explain. This research was further explored whether LIPUS can reconstruct the hematopoietic function in myelosuppression induced by paclitaxel plus carboplatin combination chemotherapy. So, in this research we triggered the hematopoietic damages of SD rats by paclitaxel plus carboplatin. We thought animal models should be conducted in strict simulation of clinical diagnosis and treatment to disease in order to be closer to the clinical status quo. ![]() In consideration of current clinical application, different cell cycle chemotherapy drugs are often used to treat cancer in combination, such as paclitaxel and carboplatin ( 19, 20). Our previous experimental study showed that LIPUS can promote the proliferation, differentiation and migration of bone marrow mesenchymal stem cells ( 14- 16), and also found that LIPUS can treat leukopenia caused by cyclophosphamide chemotherapy and elevate leukocytes count after cytarabine chemotherapy ( 17, 18).īut, the above results of our research only use single drugs to induce myelosuppression. Therefore, a safer, more effective and economical method should be found to promote medullary hematopoiesis function to support cancerous people complete the course of chemotherapy successfully and improve their quality of life.Īs an emerging physical therapy, low-intensity pulsed ultrasound (LIPUS) has been approved as a safe non-invasive method for the treatment of bone healing, soft-tissue regeneration, inhibition of inflammation, non-invasive neuromodulation, and other applications ( 13). ![]() But these methods have disadvantages for some patients such as transfusion reactions and transfusion-transmitted infections ( 10), expensive cost ( 11, 12), and so on. At present, the treatments rely mainly on leukocytes drugs ( 7), platelet transfusion ( 8) and haemopoietic growth factors ( 9) therapy to improve the basic conditions of patients. Re-establishing hematopoiesis and immune system are the key to treating these diseases. There are symptoms such as anemia, hemorrhage, infection, even life-threatening ( 6) because of the myelosuppression. The hematopoietic tissue and cell will be damaged mostly then lead to the number of peripheral blood cells released from bone marrow decreasing, especially the leukocytes decline seriously in a short time. Among these toxic and side effects, hematopoietic damage triggered off by chemotherapeutic drugs is particularly serious. Most of the chemotherapeutic drugs will kill cancer cells and normal tissue cells simultaneously, causing a variety of toxic side effects such as digestive tract reaction, central nervous system reaction, cardiovascular disease and myelosuppression ( 1- 5). Keywords: Myelosuppression low intensity pulsed ultrasound (LIPUS) hematopoiesis paclitaxel carboplatin The mechanisms may be LIPUS can increase the levels of hematopoietic regulators and adhesion molecules. ![]() control group, P<0.05).Ĭonclusions: The results indicated that LIPUS can relieve myelosuppression induced by combined treatment of paclitaxel and carboplatin. Results: The results showed in the LIPUS group the blood cells count, hematopoietic tissue of bone marrow, the colonies formed from adhering of bone marrow stromal cells, levels of hematopoietic regulators and adhesion molecules all increased (LIPUS group vs. The evaluation of blood cells counts, Hematoxylin-Eosin staining (H&E staining), scanning electron microscopy, enzyme-linked immunosorbent assay (ELISA) and real-time quantitative PCR (qPCR) was then performed. The LIPUS group was given continuous LIPUS irradiation for 7 days, while the control group was given sham irradiation (no energy output). These myelosuppressive rats were randomly divided into LIPUS group (n=40) and control group (n=40). Methods: The rat model of myelosuppression was established by continuous injection of paclitaxel and carboplatin for 4 days. To be closer to the clinical status quo, this experiment was designed to show whether it was the same effect of LIPUS on myelosuppression caused by combination therapy of chemotherapy drugs. But in clinics, chemotherapy is often performed with multiple drugs simultaneously.
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